Technical Data
Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I)
Molecular Biology Storage: -20°CShipping: Blue Ice
Insulin Like Growth Factor-I (IGF-I) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. Recombinant Human IGF-I is a single, nonglycosylated, 7.6kD protein containing 70 amino acid residues. Insulin-like growth factor 1 (IGF-I) is involved in regulation of neuronal growth and development in central and peripheral nervous system. It is known to protect neurons against cell death induced by amyloidogenic derivatives, glucose or serum deprivation through pathways involving AKT kinase and transcription factor FKHRL1 phosphorylation. Activation of the insulin-like growth factor system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like breast and thyroid cancers.

Biological Activity:
Human IGF-I is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is 1ng/ml, corresponding to a specific activity of 1x10e6 units/mg. For most in vitro applications, IGF1 exerts its biological activity in the concentration range of 0.2-20ng/ml.

Responding Cells (partial list): Bone, muscle cells, CHO cells

AA Sequence:

Storage and Stabiltiy:
Lyophilized powder may be stored at -20°C. Reconsitute in sterile dH2O, 0.1% HSA or BSA. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~95% by SDS-PAGE and HPLC analyses.
Form: Supplied as a lyophilized powder. Reconsitute in sterile ddH2O, 0.1% HSA or BSA to 0.1mg/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Zhang, H. et al., (2004) J. Biol. Chem. 279, 19683-19690. 2. Nedelkov, D. et al., (2003) FEBS Letts. 536:130-134. 3. Zhang, H. and Brodt, P. (2003) Oncogene 22:974-982.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.