Technical Data
I7661-11C
Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I) (Liquid)
100ug
500ug
Growth Factors, Cytokines Storage: -20°CShipping: Blue Ice
Insulin Like Growth Factor-I (IGF-I) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. Recombinant Human IGF-I is a single, nonglycosylated, 7.6kD protein containing 70 amino acid residues. Insulin-like growth factor 1 (IGF-I) is involved in regulation of neuronal growth and development in central and peripheral nervous system. It is known to protect neurons against cell death induced by amyloidogenic derivatives, glucose or serum
deprivation through pathways involving AKT kinase and transcription factor FKHRL1 phosphorylation. Activation of the insulin-like growth factor system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like breast and thyroid cancers

Biological Activity:
Human IGF-I is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is 1ng/ml, corresponding to a specific activity of 1x10e6 units/mg. For most in vitro applications, IGF1 exerts its biological activity in the concentration range of 0.2-20ng/ml.

Responding Cells (partial list): Bone, muscle cells, CHO cells

AA Sequence:
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
7.6kD
Source: Recombinant, Human (E. coli)
Purity: 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug)
Concentration: ~1mg/ml
Form: Supplied as a lliquid in 50mM acetic acid, 0.1% BSA.. Available as a lyophilized powder, see I7661-11.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.