Technical Data
I7661-14
Insulin-Like Growth Factor II, Recombinant, Human (Human IGF-II, IGF-II, IGFII, IGFII, IGF2, IGF-2, IGF 2)
10ug
50ug
Molecular Biology Storage: -20CShipping: RT
Insulin Like Growth Factor-II (IGF-II) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types. Human IGF-II is a 7.5kD protein containing 67 amino acid residues.

Biological Activity:
The ED50, calculated by the dose-dependent induction of MCF-7 cell proliferation was found to be 0.85ng/ml corresponding to a specific activity of 1,176,470IU/mg.

AASequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Tyr-Arg-Pro-Ser.

Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA . Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
7.5kD
Source: Recombinant Human IGF2 esxpressed in E. coli
Purity: 97% by SDS-PAGE and RP- HPLC. Purified by propietary chromatographic techniques.

Form: Supplied as a lyophilized powder without additives. Reconstitute in sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml..

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological application reference: Nedelkov, D. et al., (2003) FEBS Letts. 536:130-134.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.