Technical Data
Insulin-Like Growth Factor II, Recombinant, Human (Human IGF-II, IGF-II, IGFII, IGFII, IGF2, IGF-2, IGF 2)
Molecular Biology Storage: -20CShipping: RT
Biological Activity: Receptor binding to the IGF-II receptor in L6 myoblasts: ED50 51.14 ng/ml, as determined by an independent laboratory. At 10ng/ml this material gave a 3.4 fold stimulation of 3 H thymidine incorporation over background in chick embryo broblasts.

Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 95% by H.P.L.C.
Form: Supplied as a lyophilized powder.

Important Note: This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.
1. Lowe, W.L., In: Insulin-Like Growth Factors: Molecular and Cellular Aspects LeRoith, D., CRC Press Inc. Boca Raton, p49, 1991. 2. LeRoith, D., and Roberts, Jr., CT. Ann. New York Acad. Sci. 692: 1, 1993. 3. Francis, G.L., et al., Novel recombinant fusion protein analogues of insulin-like growth-factor (IGF)-I indicate the relative importance of IGF-binding protein and receptor binding for enhanced biological potency. J. Mol. Endocrinol. 8: 213223, 1992. 4. Francis, G.L., et al., Insulin-like growth factor (IGF)-II binding to IGF-binding proteins and IGF receptors is modi ed by deletion of the N-terminal hexapeptide or substitution of arginine for glutamate-6 in IGF-II. Biochem. J. 293: 713719, 1993.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.