Technical Data
I7661-14E
Insulin-Like Growth Factor II, Recombinant, Human (Insulin-like Growth Factor 2 (Somatomedin A), IGF-II, IGF2, C11orf43, FLJ22066, FLJ44734, PP1446, PP9974, Somatomedin-A)
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to human Insulin Like Growth Factor-II expressed in E.coli is a single, non-glycosylated, polypeptide chain.

Molecular Weight:
~7.5kD

Biological Activity:
The ED50, calculated by the competitive binding of IGF-II plasma derived to human placental membrane is less then 1.0ng/ml, corresponding to a specific activity of 106 IU/mg.

Dimers and Aggregates:
1% (SDS-PAGE)

Protein Content:
Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a standard solution of IGF-II as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~7.5kD
Purity: ~97% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: Not determined
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Overexpression of human IGF-II mRNA in the brain of transgenic mice modulates IGFBP-2 gene expression in the medulla oblongata. J Endocrinol 2004 Sep;182(3):445-55. 2. IGF and IGF-binding protein expression in the growth plate of normal, dexamethasone-treated and human IGF-II transgenic mice. J Endocrinol 2002 Oct;175(1):143-53. 3. Kinetics and regulation of site-specific endonucleolytic cleavage of human IGF-II mRNAs. Nucleic Acids Res 2001 Sep 1;29(17):3477-86. 4. A quantitative reverse transcription and polymerase chain reaction assay for human IGF-II allows direct comparison of IGF-II mRNA levels in cancerous breast, bladder, and prostate tissues. Growth Horm IGF Res 2000 Apr;10(2):61-70. 5. Measurement of human IGF-II using Sephacryl extraction: a rapid and reliable assay method. Ann Clin Biochem 1996 May;33 ( Pt 3):201-8. 6. The cis-acting elements involved in endonucleolytic cleavage of the 3' UTR of human IGF-II mRNAs bind a 50 kDa protein. Nucleic Acids Res 1996 Mar 15;24(6):1000-7.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.