Technical Data
Insulin-Like Growth Factor Binding Protein 5, Recombinant, Human (IGFBP5, IBP5, IBP-5)
Molecular Biology Storage: -20CShipping: Blue Ice
Human IGF-BP5 is a 28.6kD cysteine-rich secreted protein produced by vascular smooth muscle cells. It is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGF-I on smooth muscle cells, fibroblasts or osteoblasts. Studies show that IGF-BP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGF-BP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole body growth inhibition, and retarded muscle development. Recombinant human IGF-BP5 is a 28.6kD protein consisting of 253 amino acid residues.

Source: E.coli

Endotoxin: 0.1ng/ug

Biological Activity: The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is 0.3ug/ml, in the presence of 15ng/ml of human IGF-II.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Stable for at least 6 months at -20C. Reconstitute with sterile ddH2O, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% (SDS-PAGE and HPLC)
Concentration: Not determined
Form: Supplied as a lyophilized powder from 10mM Sodium Citrate solution, pH 3.0. Reconstitute in ddH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.