Technical Data
I7661-16X2-P
Insulin-Like Growth Factor Binding Protein 6 (IGFBP6, IGF Binding Protein 6, IBP6) (Control Peptide)
100ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
C-RPQDVNRRDQQRNP, from the internal region of human IGFBP6.
Control peptide corresponding to polyclonal antibody, Catalog#I7661-16X2, goat x human.

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Adrenal Gland lysates

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 monthsat -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking (see corresponding antibody I7661-16X2):
Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Purity: Highly Purified
Concentration: ~0.5mg/ml
Form: Supplied as a lyophilized powder in PBS.
Specificity: Recognizes human IGFBP6 at ~25kD. Species sequence homology: bovine.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Cheung C, Vesey D, Cotterill A, Douglas M, Gobe G, Nicol D, Johnson D. Altered messenger RNA and protein expressions for insulin-like growth factor family members in clear cell and papillary renal cell carcinomas. Int J Urol. 2005 Jan;12(1):17-28.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.