Technical Data
I7661-26HX
Insulin Receptor, beta (IRb) (BSA & Azide Free)
Description:
The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a b-a-a-b configuration. The b-subunit (95kD) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kD) is completely extracellular.

Applications:
Suitable for use in Affinity Purification, Conjugation, ELISA, Western Blot, Tyrosine Kinase Assay and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Affinity Purification: With peptide elution.
Tyrosine Kinase Assay: Antibody mediated capture on microtiter plates
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:50 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilution to be determined by researcher.

Positive Control:
HEP-G-2, IM-9 lymphocyte cells, placenta or brain.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13F225Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant fragment including the C-terminal 100 amino acids of human insulin receptor. Cellular Localization: Cell membrane.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins and no preservatives added. Also available with BSA and azide. See I7661-26H.
Specificity:
Recognizes human Insulin Receptor, beta (95kD). Species Crossreactivity: monkey, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.