Technical Data
I7661-26HX
Insulin Receptor, beta (IRb) (BSA & Azide Free)
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Technical Data |
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I7661-26HX |
Insulin Receptor, beta (IRb) (BSA & Azide Free) |
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Description: The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a b-a-a-b configuration. The b-subunit (95kD) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kD) is completely extracellular. Applications: Suitable for use in Affinity Purification, Conjugation, ELISA, Western Blot, Tyrosine Kinase Assay and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilutions: Affinity Purification: With peptide elution. Tyrosine Kinase Assay: Antibody mediated capture on microtiter plates Western Blot: 1-2ug/ml for 2hrs at RT Immunohistochemistry (Formalin/paraffin): 1:50 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution to be determined by researcher. Positive Control: HEP-G-2, IM-9 lymphocyte cells, placenta or brain. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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