Technical Data
Insulin Receptor, beta (IRb) (BSA & Azide Free)
The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a b-a-a-b configuration. The b-subunit (95kD) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kD) is completely extracellular.

Suitable for use in Affinity Purification, Conjugation, ELISA, Western Blot, Tyrosine Kinase Assay and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Affinity Purification: With peptide elution.
Tyrosine Kinase Assay: Antibody mediated capture on microtiter plates
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1:50 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilution to be determined by researcher.

Positive Control:
HEP-G-2, IM-9 lymphocyte cells, placenta or brain.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG13F225Affinity Purified
100ul-20CBlue IceHumanMouse
Recombinant fragment including the C-terminal 100 amino acids of human insulin receptor. Cellular Localization: Cell membrane.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins and no preservatives added. Also available with BSA and azide. See I7661-26H.
Recognizes human Insulin Receptor, beta (95kD). Species Crossreactivity: monkey, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.