Technical Data
I7661-31D
Integrin alpha1 I
Description:
Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry and Inhibition assays. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5ug/ml
Western Blot: 2ug/ml
Immunohistochemistry: 2ug/ml with paraformaldehyde fixation.
Immunoprecipitation: 1ug/ml
Flow Cytometry: 2ug/ml
Inhibition Assay: 1-5ug/ml. Using activated human lymphocyte binding to laminin, collagen IV and fibronectin.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q1420Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human Integrin alpha1 I
Purity:
Purified by Protein A Sepharose chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes the I domain (Val151Ala364) of human a1 integrin (CD49a). Precipitates proteins of Mr 180kD, pI 5.9-6.0 (alpha chain) and Mr 115kD, pI 4.5-5.0 (beta chain) from human lymphocyte lysates. Does not recognize rat recombinant I domain of integrin a1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fabbri, M. et al. (1996) Tissue Antigens 48: 4751. 2. Zocchi, M.R. et al. (1991) Electrophoresis 12: 527535