Technical Data
I7661-32C
Integrin alpha2, beta1 (Collagen Receptor, VLA2)
Description:
The epitope is dependent on the presence of the (I) domain of alpha 2 integrin subunit and does not bind VLA-2 variant which lacks the Idomain. Inhibits VLA-2 mediated cell adhesion to collagen and laminin. Does not detect alpha 2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved.

Applications:
Suitable for use in Immunoprecipitation, Flow Cytometry, Inhibition and Immunohistochemistry. Not suitable for Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation: 2-5ug for lysate of 10e6 cells
Flow Cytometry: 10ug/ml
Immunohistochemistry (frozen tissue sections): 5ug/ml
Inhibition: 10ug/ml Inhibits VLA-2 mediated cell adhesion to collagen and laminin.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2Q1003Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Integrin alpha 2, beta 1
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes the human collagen receptor VLA-2 (alpha 2 beta 1) integrin complex. Species Crossreactivity: porcine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wang, X.Q et al. Mol. Biol. of the Cell 9: 865–874 (1998).