Technical Data
I7661-37Y2
Integrin alphaM (Itgam, CD11b/CD18, CD11B (p170), CD11b Antigen, Cell Surface Glycoprotein MAC-1 alpha Subunit, Complement Component Receptor 3 alpha-a (CR3A), Complement Receptor Type 3, CR3, CR3A, CR-3 alpha Chain, F730045J24Rik, Integrin alpha-M Precur
Description:
CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is also a marker for hematopoietic/neural stem and progenitor cells and microglia.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml; Using macrophage lysates, a doublet is seen at ~95 and 103kD. A weaker doublet is also seen at ~130 and 160kD.
Immunohistochemistry (Formalin fixed paraffin embedded sections): 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide made to an internal region (within residues 500-600) of the mouse CD11 (b/c) protein (Swiss-Prot# P05555).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes mouse CD11. Species Crossreactivity: human. Species Sequence Homology: murine CD11b and CD11c - 100%; human CD11b, CD11c and CD11d - 92%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.