		Technical Data
I7661-37Y2	Integrin alphaM (Itgam, CD11b/CD18, CD11B (p170), CD11b Antigen, Cell Surface Glycoprotein MAC-1 alpha Subunit, Complement Component Receptor 3 alpha-a (CR3A), Complement Receptor Type 3, CR3, CR3A, CR-3 alpha Chain, F730045J24Rik, Integrin alpha-M Precur

Description:
CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is also a marker for hematopoietic/neural stem and progenitor cells and microglia.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml; Using macrophage lysates, a doublet is seen at ~95 and 103kD. A weaker doublet is also seen at ~130 and 160kD.
Immunohistochemistry (Formalin fixed paraffin embedded sections): 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide made to an internal region (within residues 500-600) of the mouse CD11 (b/c) protein (Swiss-Prot# P05555).

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes mouse CD11. Species Crossreactivity: human. Species Sequence Homology: murine CD11b and CD11c - 100%; human CD11b, CD11c and CD11d - 92%.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.