Technical Data
I7661-38C
Integrin, beta1, active (ITGB1, Integrin, beta 1 (Fibronectin Receptor, beta Polypeptide, Antigen CD29 includes MDF2, MSK12), CD29, Fibronectin Receptor Subunit beta, FNRB, GPIIA, MDF2, MSK12, VLA-4 Subunit beta, VLAB, VLA-BETA)
Description:
The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit non-convalently associated with the common b1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. This antibody recognizes epitopes in the 355-425 region of the common b1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli.

Applications:
Suitable for use in ELISA, Western Blot, FACS and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
FACS: 2.5ug/5x10e5 cells
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b2Q839Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human b1 integrins purified from Triton X-100 lysates of human lung, liver, and skeletal muscle tissues. Specifically selected due to its increased reactivity with T-lymphoblasts upon Mn2+ stimulation.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.1. No preservatives added.
Specificity:
Recognizes the active form of human b1 integrins. Recognizes epitopes in the 355-425 region of the common b1 subunit of VLA integrins. Does not augment the affinity of beta 1 for ligands. Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Luque, et al. 1996. J. Biol. Chem. 271:11067.