Technical Data
I7661-71A
Interferon alpha 1b, Recombinant, Human (IFNa1b)
100ug
Growth Factors, Cytokines Storage: -20°CShipping: Blue Ice
Interferon-gamma (IFN- , also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-& or the various IFN- family proteins. Mature IFN- exists as noncovalently-linked homodimers. Human IFN- is highly species specific and is biologically active only in human and primate cells.

IFN- was originally characterized based on its antiviral activities. The protein also exerts anti-proliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN- induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN- also augments TH1 cell expansion and may be required for TH1 cell differentiation.

IFN- exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites. The IFN- receptor is present on almost all cell types except mature erythrocytes and has been cloned and characterized. The IFN- receptor is structurally related to the recently cloned IL-10 receptor.

Source:
E. coli

Biological Activity:
Bioactivity is detected using WISH cell (a heteroploid human amnion cell line) as dependent cell strain. The specific activity shall be not less than 1.5×10e7 IU/mg of protein.

Endotoxin: 1EU/ug (LAL)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Reconstitute with sterile ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 1 month at 4 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~17kD
 Source: E. coli
Purity: 98% as determined by SDS-PAGE and HPLC.
Form: Supplied as a lyophilized powder from 0.2um sterile-filtered solution in PBS, pH 7.0, 50ug of HSA per 1ug of cytokine. Reconstitute with sterile, dH2O to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.