Interferon alpha A (alpha 2a), Recombinant, Human (IFNaA, IFNa2a) (Human 293 Cells)
|Growth Factors, Cytokines||Storage: -20°CShipping: Blue Ice|
Human Cell Expression Cytokines:
Expression of proteins in our Human 293-cell expression system is a cost-effective and scalable production method for high-authenticity, recombinant human proteins.
Functional activities of human proteins depend on proper folding, phosphorylation, disulfide bridge formation, and proteolytic processing or appropriate glycosylation. Ideally, recombinant human proteins are produced in human cells that are more authentic in terms of both physical properties and biochemical functions. However, the current process of human cell expression requires a large quantity of DNA and medium supplemented with bovine serum. Hence, it is often a daunting task to produce sufficient amount of high quality proteins for drug discovery or diagnostic development (1-100mg) at acceptable cost. United States Biological is now supplying a high yield, cost-effective and versatile system to produce active recombinant human proteins in a human cell line to meet the special needs of the broad scientific community and drug discovery industry. In this system, we have broken key bottleneck steps to increase protein production and reduce cost, including
• Engineered human cell line adapted to suspension for growth in serum-free and chemically defined medium
• Proprietary expression vectors driven by strong promoters and signal peptides
• Large scale transient expression with small quantity of DNA
• Rapid creation of stable human cell lines and adaptation to serum-free medium
• Short time to scale up production
• Efficient tag or tag-free purification
IFN alpha 2A is a glycosylated monomer with an apparent molecular mass of ~16kD.
Recombinant Human IFN alpha 2A expressed in human 293 cells.
The specific activity was determined by the dose-dependent cytotoxicity of the human TF-I cell line (human erythroleukemic indicator cell line).
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% endotoxin-free recombinant HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: HEK 293 cells
Purity: ~ 95% as determined by SDS-PAGE
Endotoxin: 1EU/ug (LAL)
Form: Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS, 0.1% endotoxin-free recombinant HSA.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Mire-Sluis, A. R., et al (1996) J Immunol Methods 195: 55-61. 2. Bogdan, C., et al (2004) Immunol Rev 202: 33-48.