Technical Data
I7662-10A3
Interferon alpha A (alpha 2a), Recombinant, Human (IFNaA, IFNa2a)
100ug
Growth Factors, Cytokines Storage: -20°CShipping: Blue Ice
Human Interferon-alpha 2a (IFN-±2a) is a single polypeptide chain of 165 amino acid residues, containing 4 Cys to form two disulfide bonds at positions of Cys1-Cys29 and Cys98-Cys138 and it has a molecular mass of approximately 19kD.

IFN-± can exert certain cell activities through binding to specific cell surface receptors, as firstly induce to develop special proteins, such as protein kinase and 2, 5-oligo polyadenylic acid synthase etc. The unique character of two enzymes is that they can be activated respectively by double chains RNA to produce the effect of self-phosphorylation. IFN can also enhance phagocytosis activity of macrophage and special toxicity of lymphocyte to target cells to cause immunoregulation.

Its biological effects contains: (1) Anti-virus: IFN-± has broad-spectrum anti-virus effect; (2) Cytostatic effect to certain cells; (3) immunoregulation effect: accelerates the expression of MHC antigen by most cells and active NK cells as well as CTL. (4) Repress and kill tumor cells: IFN-± kills the tumor cells through accelerating the immunological function and enhancing the killing ability of macrophage, NK and CTL.

Source:
Escherichia coli.

Biological Activity:
Bioactivity is detected using cytopathic effect inhibition assay method, WISH cell (a heteroploid human amnion cell line) as dependent cell strain. The specific activity shall be not less than 1.0×10e8 IU/mg.

Endotoxin: 1EU/ug (LAL)

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Reconstitute with sterile ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 1 month at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~19.2kD
 Source: E. coli
Purity: 97% as determined by SDS-PAGE and HPLC.
Form: Supplied as a lyophilized powder from 0.2um sterile-filtered solution in PBS, pH 7.0, 50ug of HSA per 1ug of cytokine. Reconstitute with sterile, dH2O to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.