Technical Data
I7662-14Q
Interferon beta 1 (beta 1a), Recombinant, Human (BSA Free) (IFNb1, IFNb1a)
100KU
Growth Factors, Cytokines Storage: -70CShipping: Dry Ice
One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1 . In contrast to IFN-alpha, IFN-beta is strictly species-specific. IFN-beta of other species is inactive in human cells. IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. IFN-beta increases the expression of HLA class I antigens and blocks the expression of HLA class II antigens stimulated by IFN-gamma.

Recombinant Human Interferon-Beta 1a is a single, glycosylated polypeptide chain containing one N-glycosylation site, 166 amino acids and having a molecular mass of ~23kD

Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.

Accession Number: V00534

Specific Activity: ~1x10e8u/mg

Biological Activity:
Interferon Beta-1a is fully biologically active when compared to standard. The specific activity as determined in the cytopathic effect inhibition assay as described [Rubinstein, et al.. In this antiviral assay for interferon about 1u/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon beta (Hu-IFN-b) provided by the National Institutes of Health. Unit of activity was measured on VERO cells with vesicular stomatitis virus (VSV).

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Molecular Weight:
~23kD
Source: Human, recombinant cDNA obtained from human fibroblast mRNA expressed in CHO cells ]
Purity: Chromatographically purified ( 95%). Endotoxin: 0.1ng/ug (IEU/ug).
Concentration: ~10e6u/ml
Form: Supplied as a liquid 50mM acetic acid, 0.1% BSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Rubinstein, S., Familletti, P.C., Pestka, S., Convenient Assay for Interferons, J. Virol. 37: 755-758 (1981). 2. Familletti, P.C., Rubinstein, S., Pestka, S., A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon, in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394 (1981). 3. Pestka, S., Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119: 14-23 (1986).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.