Technical Data
Interferon beta 1 (beta 1a), Recombinant, Human (IFNb1, IFNb1a)
Molecular Biology Storage: -20CShipping: RT
One of the type I interferons produced by fibroblasts in response to stimulation by live or inactivated virus or by double-stranded RNA. It is a cytokine with antiviral, antiproliferative, and immunomodulating activity. The synthesis of IFN-beta can be induced by common inducers of interferons, including viruses, double-stranded RNA, and micro-organisms. It is induced also by some cytokines such as TNF and IL-1 . In contrast to IFN-alpha, IFN-beta is strictly species-specific. IFN-beta of other species is inactive in human cells. IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. IFN-beta increases the expression of HLA class I antigens and blocks the expression of HLA class II antigens stimulated by IFN-gamma.

Recombinant Human Interferon-Beta 1a is a single, glycosylated polypeptide chain containing 166 amino acids and having a molecular mass of 22500D.

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Asn-Leu-Leu.

Biological Activity:
Interferon Beta-1a is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 270 x 10e6 IU/ mg.

Dimers and Aggregates: < 1% (by silver-stained SDS-PAGE).

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a standard solution of IFN-beta as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Molecular Weight:
Source: Recombinant, Human (CHO cells)
Purity: Chromatographically purified (> 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain). Endotoxin: 0.1ng/ug (IEU/ug).
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder from 91mg Human albumin, 620mg mannitol, 9mg sodium acetate.
Reconstitute with sterile ddH2O to a concentration of .0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Debat Zoguereh, D., et al., Rev. Neurol. (Paris) 160(11): 1081-1084 (2004). 2. Kalanie, H., et al., Eur. Neurol. 52(4): 202-206 (2004). 3. Filippi, M., et al., Lancet 364(9444): 1489-1496 (2004). 4. Lim, E.T., et al., J. Negat. Results Biomed. 3(1): 4 (2004). 5. Villa, A.M., et al., Arq. Neuropsiquiatr. 62(3B): 892-894 (2004). 6. Prosser, L.A., et al., Value Health 7(5): 554-568 (2004).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.