Technical Data
Interferon gamma, Recombinant, Human (IFNg)
Molecular Biology Storage: -70CShipping: Dry Ice
Interferon-gamma (IFN- , also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-b or the various IFN-a family proteins. Mature IFN-y exists as noncovalently-linked homodimers. Human IFN-y is highly species specific and is biologically active only in human and primate cells.
IFN-y was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-y induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-y also augments TH1 cell expansion and may be required for TH1 cell differentiation.

Biological Activity:
Fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay is less then 0.05 ng/ml, corresponding to a specific activity of 2.0 x 10e7 IU/ mg.

Endotoxin: 1EU/ug as determined by LAL method.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Molecular Weight:
17 kD
Source: Escherichia coli
Purity: ~95% by SDS-PAGE and HPLC analyses.
Concentration: 1mg/ml
Form: Supplied as a lyophilized powder in PBS, pH 7.5. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.