Technical Data
I7662-17D1
Interferon gamma, Recombinant, Mouse (IFNg)
20ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
The major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced, among other things, by Interleukin-2 , FGF-basic , and EGF .
Recombinant Murine IFN-gamma produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6kD.
Recombinant IFN-gamma is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC

Biological Activity:
The specific activity as determined in a viral resistance assay is <0.1ng/ml, corresponding to a specific activity of 1x10e7U/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Formula:
N/A

Molecular Weight:
15.60kD
Source: E. coli
Purity: 95% as determined by RP-HPLC and SDS-PAGE Silver Stained gel. Chromatographically purified.
Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.