Technical Data
I7662-18F
Interferon gamma, carrier-free, Recombinant, Mouse (IFNg)
10ug
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Technical Data |
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I7662-18F |
Interferon gamma, carrier-free, Recombinant, Mouse (IFNg) |
10ug |
| Growth Factors, Cytokines | Storage: -70°CShipping: Dry Ice |
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Background: Assay Used to Measure Bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for mouse interferon gamma (mouse IFN-g) provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. Unit of activity measured on mouse L929 cells with encephalomyocarditis virus (EMCV). Specific Activity: ~1x10e7units/mg Storage and Stability: Product should be kept at -70°C or below for retention of full activity. When thawing, the contents should be aliquoted to keep freezing and thawing to a minimum. Molecular Weight: 15,521 |
Source: cDNA obtained from mouse leukocytes expressed in E. coli Purity: 95% Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Rubinstein, S., Familletti, P.C., Pestka, S., Convenient Assay for Interferons, J. Virol. 37: 755-758 (1981). 2. Familletti, P.C., Rubinstein, S., Pestka, S., A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon, in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394 (1981). 3. Pestka, S., Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119: 14-23 (1986).
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