Technical Data
I7662-18F
Interferon gamma, carrier-free, Recombinant, Mouse (IFNg)
10ug
Molecular Biology Storage: -70CShipping: Dry Ice
Background:
Assay Used to Measure Bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for mouse interferon gamma (mouse IFN-g) provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. Unit of activity measured on mouse L929 cells with encephalomyocarditis virus (EMCV).

Specific Activity:
~1x10e7units/mg

Storage and Stability:
Product should be kept at -70C or below for retention of full activity. When thawing, the contents should be aliquoted to keep freezing and thawing to a minimum.

Molecular Weight:
15,521
Source: cDNA obtained from mouse leukocytes expressed in E. coli
Purity: 95%
Concentration: ~0.2mg/ml
Form: Supplied as a liquid in PBS, pH 7.2. No preservative added.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Rubinstein, S., Familletti, P.C., Pestka, S., Convenient Assay for Interferons, J. Virol. 37: 755-758 (1981). 2. Familletti, P.C., Rubinstein, S., Pestka, S., A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon, in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394 (1981). 3. Pestka, S., Interferon Standards and General Abbreviations, in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119: 14-23 (1986).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.