Technical Data
I7662-19R
Interferon-inducible T Cell Alpha Chemoattractant, Recombinant, Human (I-TAC)
10ug
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Technical Data |
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I7662-19R |
Interferon-inducible T Cell Alpha Chemoattractant, Recombinant, Human (I-TAC) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Source: Recombinant corresponding to human Interferon-inducible T Cell Alpha Chemoattractant expressed in E. coli. Molecular Weight: ~8kD Endotoxin: 0.1ng/ug Activity: ED50 range =1.0-10 ng/ml, determined by dose dependent chemotaxis with human lymphocytes cultured in the presence of IL-2. The optimal concentration for each specific application should be determined by an initial dose response assay. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~8kD |
Source: E. coli Purity: ~95% (SDS PAGE) Concentration: Not determined Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Cole, K.E., C.A. Strick, T.J. Paradis, K.T. Ogborne, M. Loetscher, R.P. Gladue, W. Lin, J.G. Boyd, B. Moser, D.E. Wood, B.G. Sahagan, and K. Neote (1998) Interferoninducible T cell alpha chemoattractant (I-TAC): a novel non-ELR CXC chemokine with potent activity on activated T cells through selective high affinity binding to CXCR3. J. Exp. Med. 187:2009-2021. 2. Laich, A., M. Meyer, E.R. Werner, and G. Werner-Felmayer (1999) Structure and expression of the human small cytokine B subfamily member 11 (SCBY11/formerly SCYB9B, alias I-TAC) gene cloned from IFN-gamma-treated human monocytes (THP- 1). J. Interferon Cytokine Res. 19:505-513. 3. Cox, M.A., C.H. Jenh, W. Gonosiorek, J. Fine, S.K. Narula, P.J. Zavodny, and R.W. Hipkin (2001) Human interferon-inducible 10-kDa protein and human interferoninducible T cell alpha chemoattractant are allotopic ligands for human CXCR3: differential binding to receptor states. Mol. Pharmacol. 59:707-715. 4. Sauty, A., R.A. Colvin, L. Wagner, S. Rochat, F. Spertini, and A.D. Luster (2001) CXCR3 internalization following T cell-endothelial cell contact: preferential role of IFN-inducible T cell alpha chemoattractant (CXCL11). J. Immunol. 167:7084-7093. 5. Hensenbergen, P.J., E.M. van der Raaij-Helmer, R. Dijkman, R.C. van der Schors, G. Werner-Felmayer, D.M. Boorsma, R.J. Scheper, R. Willemze, and C.P. Tensen (2001) Processing of natural and recombinant CXCR3-targeting chemokines and implications for biological activity. Eur. J. Biochem. 268:4992-4999.
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