Technical Data
Interferon-inducible T Cell Alpha Chemoattractant, Recombinant, Human (I-TAC)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant corresponding to human Interferon-inducible T Cell Alpha Chemoattractant expressed in E. coli.

Molecular Weight:

Endotoxin: 0.1ng/ug

ED50 range =1.0-10 ng/ml, determined by dose dependent chemotaxis with human lymphocytes cultured in the presence of IL-2. The optimal concentration for each specific application should be determined by an initial dose response assay.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~95% (SDS PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Cole, K.E., C.A. Strick, T.J. Paradis, K.T. Ogborne, M. Loetscher, R.P. Gladue, W. Lin, J.G. Boyd, B. Moser, D.E. Wood, B.G. Sahagan, and K. Neote (1998) Interferoninducible T cell alpha chemoattractant (I-TAC): a novel non-ELR CXC chemokine with potent activity on activated T cells through selective high affinity binding to CXCR3. J. Exp. Med. 187:2009-2021. 2. Laich, A., M. Meyer, E.R. Werner, and G. Werner-Felmayer (1999) Structure and expression of the human small cytokine B subfamily member 11 (SCBY11/formerly SCYB9B, alias I-TAC) gene cloned from IFN-gamma-treated human monocytes (THP- 1). J. Interferon Cytokine Res. 19:505-513. 3. Cox, M.A., C.H. Jenh, W. Gonosiorek, J. Fine, S.K. Narula, P.J. Zavodny, and R.W. Hipkin (2001) Human interferon-inducible 10-kDa protein and human interferoninducible T cell alpha chemoattractant are allotopic ligands for human CXCR3: differential binding to receptor states. Mol. Pharmacol. 59:707-715. 4. Sauty, A., R.A. Colvin, L. Wagner, S. Rochat, F. Spertini, and A.D. Luster (2001) CXCR3 internalization following T cell-endothelial cell contact: preferential role of IFN-inducible T cell alpha chemoattractant (CXCL11). J. Immunol. 167:7084-7093. 5. Hensenbergen, P.J., E.M. van der Raaij-Helmer, R. Dijkman, R.C. van der Schors, G. Werner-Felmayer, D.M. Boorsma, R.J. Scheper, R. Willemze, and C.P. Tensen (2001) Processing of natural and recombinant CXCR3-targeting chemokines and implications for biological activity. Eur. J. Biochem. 268:4992-4999.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.