Technical Data
I7662-21
Interferon gamma, Recombinant, Human (IFNg)
100ug
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Technical Data |
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I7662-21 |
Interferon gamma, Recombinant, Human (IFNg) |
100ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Human interferon-gamma (hIFN-gamma) is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Natural human interferon-gamma is composed of 143 amino acid residues without cysteine residues and is glycosylated. Recombinant human interferon gamma was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Additional amino acid (Methionine) is attached at N-terminus. Molecular Weight: 16.9kD (144 amino acids) Sequence: MQDPYVKEAE/ NLKKYFNAGH/ SDVADNGTLF/ LGILKNWKEE/ SDRKIMQSQI/ VSFYFKLFKN/ FKDDQSIQKS/ VETIKEDMNV/ KFFNSNKKKR/ DDFEKLTNYS/ VTDLNVQRKA/ IHELIQVMAE/ LSPAAKTGKR/ KRSQMLFRGR/ RASQ Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Endotoxin Level: < 1.0EU/1ug protein (LAL method) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 16.9kD |
Source: Recombinant, Human, E. coli Purity: 95% by SDS PAGE Concentration: ~1mg/ml Form: Supplied as a liquid in 20mM Tris, pH 7.4. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Sen, G.C. & Lengyel, P., J. Biol. Chem. 267(8): 5017-5020 (1992). 2. Arakawa, T. & Hsu, Y.-R., Biochemistry 26: 5428-5432 (1987). 3. Rinderknecht, E., et al., J. Biol. Chem. 259(11): 6790-6797 (1984).
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