Technical Data
I7662-21
Interferon gamma, Recombinant, Human (IFNg)
100ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Human interferon-gamma (hIFN-gamma) is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Natural human interferon-gamma is composed of 143 amino acid residues without cysteine residues and is glycosylated. Recombinant human interferon gamma was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Additional amino acid (Methionine) is attached at N-terminus.

Molecular Weight:
16.9kD (144 amino acids)

AA Sequence:
MQDPYVKEAE/ NLKKYFNAGH/ SDVADNGTLF/ LGILKNWKEE/ SDRKIMQSQI/ VSFYFKLFKN/ FKDDQSIQKS/ VETIKEDMNV/ KFFNSNKKKR/ DDFEKLTNYS/ VTDLNVQRKA/ IHELIQVMAE/ LSPAAKTGKR/ KRSQMLFRGR/ RASQ

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
16.9kD
Source: E. coli
Purity: 95% by SDS PAGE
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.4. Endotoxin:
1.0EU/1ug protein (LAL method).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Sen, G.C. & Lengyel, P., J. Biol. Chem. 267(8): 5017-5020 (1992). 2. Arakawa, T. & Hsu, Y.-R., Biochemistry 26: 5428-5432 (1987). 3. Rinderknecht, E., et al., J. Biol. Chem. 259(11): 6790-6797 (1984).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.