Technical Data
I7662-81F
IRF1 (Interferon Regulatory Factor 1, IRF-1, MAR)
Description:
IRF1 (interferon regulatory factor 1) is a 45kD member of the IRF family of proteins. It is induced by TLR9 and INF-g stimulation, and positively regulates the expression of IFN-b, iNOS and 1L-12 p35. Human IRF1 is 325aa in length. It contains an N-terminal DNA-binding basic region aa1-140 and an acidic C-terminus aa141-325. Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11aa substitution for the C-terminal 86aa. Over aa171-325, human IRF1 shares 83aa identity with both canine and porcine IRF1.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2B11n89Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant corresponding to aa147-325 from human IRF1 expressed in E. coli (P10914).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Specificity:
Recognizes human IRF1. No cross-reactivity with recombinant human IRF2 (aa151-349), IRF3 (aa206-427), IRF4 (aa130-451), IRF5 (aa214-395), IRF6 (aa176-366), IRF7 (aa289-503), IRF8 (aa255-426), IRF9 (aa238-393), or recombinant mouse IRF1 (aa147-329).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.