Technical Data
I7662-86G
Interferon Regulatory Factor 3, NT (IRF3)
Description:
A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF3 gene encodes a 50kD protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF1 binding site PRD-I. Overexpression of IRF3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. The high amino acid homology between IRF3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF3 is expressed constitutively in a variety of tissues, and the relative levels of IRF3 mRNA do not change in virus-infected or IFN-treated cells (2). In uninfected cells, the IRF3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF3 is dependent on a nuclear export signal, and IRF3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF3 accumulates in the nucleus where it associates with CBP and p300 (3).

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:20
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG9H412Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to residues surrounding the N-terminus of human IRF3.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human Interferon Regulatory Factor 3, NT at ~51kD. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Au, WC Proc Natl Acad Sci 92(25):11657-61, 1995. 2. Lin R, et al. Mol Cell Biol 18(5):2986-96, 1998. 3. Kumar KP, et al. Mol Cell Bio 20(11):4159-68, 2000.