Technical Data
I7663-01A
Interleukin 1 alpha, Recombinant, Rat (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)
2ug
10ug
10ug
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Technical Data |
|
I7663-01A |
Interleukin 1 alpha, Recombinant, Rat (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF) |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant rat IL-1 alpha is a 17.7 kD protein containing 155 amino acid residues. Purity: 98% by SDS-PAGE and HPLC analyses Form: The sterile ltered solution was lyophilized from 0.5x Tris buffer saline, pH 8.0. Endotoxin: 0.1ng/ug (1EU/ug) Biological Activity: The ED50 as determined by the dose-dependent proliferation of murine D10S cells is < 0.005ng/ml, corresponding to a speci c activity of > 2 x 109units/mg. AA Sequence : MAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS Stability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted IL-1 alpha should be stored in working aliquots at-20°C. Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1–1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or-20°C for future use. Country of Origin : USA |
Source: E. coli Concentration: As reported Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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