Technical Data
I7663-03M
IL1A (Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1, IL1F1)
Description:
Interleukin 1 alpha is synthesized as a 31kD promolecule that does not contain a signal sequence. Following proteolytic cleavage, the mature 17kD molecule is released as a result of cell injury or apoptosis. IL-1 alpha stimulates bone osteoclast activity, has a role in the modulation of extracellular fluid calcium homeostasis and apoptosis, activates T and B lymphocytes and NK cells, and functions in development. Movement of the IL-1 alpha promolecule to the nucleus in response to endotoxin where it augments transcription of proinflammatory genes has also been documented.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:400
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2aAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant corresponding to human IL1A expressed in E. coli strain BL21 (DE3).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.03% sodium azide.
Specificity:
Recognizes human IL1A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.