		Technical Data
I7663-07N	Interleukin 10, Recombinant, Human (IL-10)	2ug 10ug

Growth Factors, Cytokines

Storage: -70°CShipping: Dry Ice

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of a-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 . IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells . IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kD noncovalently associated homodimer . The IL-10 dimer binds to two IL-10 Ra/IL-10R1 chains, resulting in recruitment of two IL-10 Rb/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 . IL-10Rb does not bind IL-10 by itself but is required for signal transduction . IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation . It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.

Biological Activity:
Determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED50 was found to be 2ng/ml, corresponding to a specific activity of 5 x 10e5 units/mg.

Endotoxin: 1EU/ug as determined by LAL method.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Molecular Weight:
18.6kD

Source: Escherichia coli
Purity: ~95% by SDS-PAGE and HPLC analyses.
Form: Supplied as a lyophilized powder in PBS, pH 7.5. Reconstitute in sterile ddH2O or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1mg/ml.
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Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.