Technical Data
I7663-17A
Interleukin 1 beta, Recombinant, Mouse (IL-1b)
2ug
10ug
10ug
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Technical Data |
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I7663-17A |
Interleukin 1 beta, Recombinant, Mouse (IL-1b) |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Murine macrophages display a transition from IL-1 beta to IL-1 alpha production during maturation of monocytes into inflammatory macrophages. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha, IFN-alpha, IFN-beta and IFN-g and also by bacterial endotoxins, viruses, mitogens, and antigens. In human skin fibroblasts IL-1 alpha and TNF-alpha induce the synthesis of IL-1beta. In pheochromocytoma cells, NGF induces the synthesis of IL-1. Recombinant Murine IL-1 is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17.5kD. Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Arg. Biological Activity: Murine Interleukin-1 beta is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of murine D10S cells < 0.002ng/ml, corresponding to a Specific Activity of 5 x10e9 IU/mg. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: Murine, recombinant protein expressed in E. coli Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: Less than 0.1ng/ug (IEU/ug). Form: Supplied as a lyophilized powder in 5mM Tris, pH 8.0, sodium phosphate dibasic.Reconstitute with sterile dH2O, 0.1 HSA or BSA to 0.1mg/mL Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Read LM, Mol Cell Biol Jan;15(1):112-9(1995). 2. Godambe SA, Chaplin DD, Bellone CJ, J Immunol Jul 1;153(1):143-52(1994) 3. Godambe SA, Chaplin DD, Bellone CJ, DNA Cell Biol Jun;13(6):561-9 (1994). 4. Lischwe MA, Newton RC, Yates RA, Protein Expr Purif Oct;4(5):499-502(1993). 5. Newton RC, Dowling R, Culp S, J Immunol Methods May 26;161(2):257-64 (1993).6. . van Oostrum J, Priestle JP, Schmitz A, J Struct Biol Oct;107(2):189-95. (1991).
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