Technical Data
I7663-17A
Interleukin 1 beta, Recombinant, Mouse (IL-1b)
2ug
10ug
Molecular Biology Storage: -20CShipping: RT
A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Murine macrophages display a transition from IL-1 beta to IL-1 alpha production during maturation of monocytes into inflammatory macrophages. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha, IFN-alpha, IFN-beta and IFN-g and also by bacterial endotoxins, viruses, mitogens, and antigens. In human skin fibroblasts IL-1 alpha and TNF-alpha induce the synthesis of IL-1beta. In pheochromocytoma cells, NGF induces the synthesis of IL-1.

Recombinant Murine IL-1 is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17.5kD.

Amino Acid Sequence:
MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQGEPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRFVFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS

Biological Activity:
Murine Interleukin-1 beta is fully biologically active when compared to standard. The ED50 is <2pg/ml, corresponding to a Specific Activity of 5x10e8 IU/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C after receipt . Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
17.5kD
Source: E. coli
Purity: 98% by RP-HPLC, SDS-PAGE. Chromatographically purified.
Form: Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with sterile ddH2O to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Read LM, Mol Cell Biol Jan;15(1):112-9(1995). 2. Godambe SA, Chaplin DD, Bellone CJ, J Immunol Jul 1;153(1):143-52(1994) 3. Godambe SA, Chaplin DD, Bellone CJ, DNA Cell Biol Jun;13(6):561-9 (1994). 4. Lischwe MA, Newton RC, Yates RA, Protein Expr Purif Oct;4(5):499-502(1993). 5. Newton RC, Dowling R, Culp S, J Immunol Methods May 26;161(2):257-64 (1993).6. . van Oostrum J, Priestle JP, Schmitz A, J Struct Biol Oct;107(2):189-95. (1991).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.