Technical Data
I7663-20B
IL1B, ID (Interleukin-1 beta, IL-1 beta, Catabolin, IL1F2)
Description:
IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.

Applications:
Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to a portion of amino acids within aa154-183 from the center region of human IL1B, conjugated to KLH.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human IL1B at ~30kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yu,J., et.al., Am. J. Gastroenterol. (2009). 2. Ito,A., et.al., J. Biol. Chem. 271(25), 14657-14660 (1996).