Technical Data
IL1B, ID (Interleukin-1 beta, IL-1 beta, Catabolin, IL1F2)
IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.

Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to a portion of amino acids within aa154-183 from the center region of human IL1B, conjugated to KLH.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human IL1B at ~30kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yu,J.,, Am. J. Gastroenterol. (2009). 2. Ito,A.,, J. Biol. Chem. 271(25), 14657-14660 (1996).