Technical Data
I7663-23
Interleukin 1 beta, Recombinant, Rat (IL-1b)
2ug
10ug
10ug
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Technical Data |
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I7663-23 |
Interleukin 1 beta, Recombinant, Rat (IL-1b) |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Interleukin-1beta (IL-1beta) is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Rat IL-1 is a 17.3kD protein containing 153 amino acid residues. Source: E. coli Reconstitution: Lyophilized rat IL-1beta is soluble in water and most aqueous buffers. The lyophilized protein can be reconstituted in water to a concentration of 0.1-1.0mg/ml. This solution can be diluted into other buffered solutions or stored at- 20°C for future use. Biological Activity: The ED50 of Rat IL-1beta as determined by the dose-dependent stimulation of murine D10S cells is 0.1ng/ml, corresponding to a specific activity of 1x10e7 units/mg. AA Sequence: MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile ddH2O, protein carrier. Aliquot and store at -20°C. Reconstituted protein is stable for 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 17.3kD |
Source: E. coli Purity: 98% by SDS-PAGE and HPLC analyses Endotoxin: 0.1ng/ug Form: Supplied as a lyophilized powder from sterile-filtered 4mM Tris, pH 8.0, without additives. Reconstitute with sterile dH2O, carrier protein to concentration of 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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