Technical Data
I7663-23
Interleukin 1 beta, Recombinant, Rat (IL-1b)
2ug
10ug
Growth Factors, Cytokines Storage: -20CShipping: RT
Interleukin-1beta (IL-1beta) is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Rat IL-1 is a 17.3kD protein containing 153 amino acid residues.

Source: E. coli

Reconstitution: Lyophilized rat IL-1beta is soluble in water and most aqueous buffers. The lyophilized protein can be reconstituted in water to a concentration of 0.1-1.0mg/ml. This solution can be diluted into other buffered solutions or stored at- 20C for future use.

Biological Activity:
The ED50 of Rat IL-1beta as determined by the dose-dependent stimulation of murine D10S cells is 0.1ng/ml, corresponding to a specific activity of 1x10e7 units/mg.

AA Sequence: MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile ddH2O, protein carrier. Aliquot and store at -20C. Reconstituted protein is stable for 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
17.3kD
Source: E. coli
Purity: 98% by SDS-PAGE and HPLC analyses Endotoxin: 0.1ng/ug
Form: Supplied as a lyophilized powder from sterile-filtered 4mM Tris, pH 8.0, without additives. Reconstitute with sterile dH2O, carrier protein to concentration of 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.