Technical Data
I7663-23A
Interleukin 1 beta, Recombinant, Rat (IL-1b)
2ug
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha , IFN-alpha , IFN-beta and IFN-g and also by bacterial endotoxins , viruses, mitogens, and antigens. In human skin fibroblasts IL-1 alpha and TNF-alpha induce the synthesis of IL-1beta . In pheochromocytoma cells, NGF induces the synthesis of IL-1.

Description:
Recombinant Rat Interleukin-1 beta produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17415.82 Dalton. The Recombinant Rat IL-1b is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Arg.

Dimers and Aggregates:
Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
The ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x 107 IU/mg.

Endotoxin:
0.1ng/ug (IEU/ug) of Recombinant Rat Interleukin-1 beta.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.558 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Rat IL-1b as a Reference Standard.

Gene:
Name: Il1b

Solubility:
It is recommended to reconstitute the lyophilized Rat Interleukin-1b in sterile 18M -cm H2O not less than 100g/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Rat IL-1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-1 beta should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Molecular Weight:
17.42kD
Source: E. coli
Purity: 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder in 10mM sodium citrate, pH 3.0.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Crocetin Inhibits mRNA Expression for Tumor Necrosis Factor-{alpha}, Interleukin-1{beta}, and Inducible Nitric Oxide Synthase in Hemorrhagic Shock.
JPEN J Parenter Enteral Nutr 2006 Jul-Aug;30(4):297-301
2. Interleukin-1 beta contributes to the upregulation of kappa opioid receptor mrna in dorsal root ganglia in response to peripheral inflammation.
Neuroscience 2006 Jun 16;
3. MyD88 is a Key Mediator of Anorexia, But Not Weight Loss, Induced by Lipopolysaccharide (LPS) and Interleukin-1{beta} (IL1{beta}).
Endocrinology 2006 Jun 15;
4. Centrally administered interleukin-1 beta sensitizes to the central pressor action of angiotensin II.
Brain Res 2006 Jun 8;
5. CpG DNA modulates interleukin 1 beta-induced interleukin-8 expression in human bronchial epithelial (16HBE14o-) cells.
Respir Res 2006 Jun 1;7(1):84
6. Histone deacetylase inhibitors prevent exocytosis of interleukin-1{beta}-containing secretory lysosomes: role of microtubules.
Blood 2006 May 9;

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.