Technical Data
Interleukin 1 Receptor Antagonist, Recombinant, Human (IL-1RA, IL1RA, IL-1ra3, IL-1RN, IL1RN, Anakinra, DIRA, ICIL-1RA, IL1 Inhibitor, IL1F3, IRAP, MGC10430, MVCD4)
Molecular Biology Storage: -20CShipping: Blue Ice
Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring protein which inhibits the activity of IL-1 alpha and IL-1 beta by competitively blocking binding to cell surface receptors for IL-1.

Suitable for use in Functional Assays. Other applications not tested.

Endotoxin: 0.1ng/ug

Biological Activity:
In an assay of dose dependent inhibition of IL-1 stimulation of D10S cells, the ED50 was expectedto be 20-40ng/ml.

Recommended Dilution:
Functional Assays: 20-40ng/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Recombinant corresponding to human IL-1RA produced in E. coli
Purity: ~98% (SDS PAGE).
Form: Supplied as a lyophilized powder from TBS. Reconstitute with 200ul sterile dH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.