Technical Data
I7663-25N3
Interleukin 1 Receptor Antagonist, Recombinant, Human (IL-1RA)
50ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
One of the most potent pro-inflammatory mediators is the early-acting cytokine interleukin-1 (IL-1), whose actions are regulated by the structurally related IL-1 receptor antagonist (IL-1 RA). IL-1 RA is a competitive IL-1 inhibitor and a powerful anti-inflammatory agent. Also, Several autoimmune and inflammatory diseases have been associated with an allelic polymorphism of the IL-1 RA gene. Recombinant human IL-1 RA was expressed in E. coli and consists of 153 amino acids.

AA Sequence:
MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
17.2kD
Source: Human, Recombinant, E. coli
Purity: ~95% by SDS-PAGE. Purified by using conventional chromatography techniques.
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.4.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Capper, et al. Clin. Exp. Immunol. 138: 348 (2004). 2. Roberge, C.J., et al., Journal of Immunology 152: 5485-5494 (1994). 3. Carter, D.B., et al., Nature 344: 633-637 (1990).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.