Technical Data
I7663-47A
Interleukin 3 Receptor alpha (IL-3RA, IL-3R alpha, IL-3R, IL3RAX, IL3RX, IL3RAY, IL3RY, CD123, MGC34174)
Description:
IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:10-1:1000 Use 1ug to label 1x10e6 cells in 100ul
Western Blot: 1:100-1:2000 Detects a band at ~70kD
Immunohistochemistry: 1:10-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k8L446Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
IL3R transfected COS cells. Cellular Localization: Type I membrane protein.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human IL3RA at ~70kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Sun Q et al. Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist. Blood 87:83-92 (1996).