Technical Data
I7663A
Interleukin 1 alpha, Recombinant, Human (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)
2ug
10ug
10ug
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Technical Data |
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I7663A |
Interleukin 1 alpha, Recombinant, Human (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF) |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins. The synthesis of IL-1 can be induced by other cytokines including TNF-alpha, IFN-alpha, IFN-beta and IFN-g and also by bacterial endotoxins, viruses, mitogens, and antigens. In human skin fibroblasts IL1-alpha and TNF-alpha induce the synthesis of IL1-beta. In pheochromocytoma cells, NGF induces the synthesis of IL1. Recombinant Human IL-1 is a single, non-glycosylated, Polypeptide chain containing 159 amino acids and having a molecular mass of 18022 Dalton. Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Pro-Phe-Ser. Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: rHuIL-1alpha is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of murine D10S cells is less then 0.001ng/ml, corresponding to a Specific Activity of 1 x 10e6 IU/mg. Endotoxin: 0.1ng/ug (IEU/ug) Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of chicken IL-1alpha as a Reference Standard. Reconstitution: Reconstitute the lyophilized rHuIL-1alpha in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Storage and Stability: Lyophilized powder is stable for at least 12 months at -20°C Reconstituted product must be stored in aliquots at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: E. coli Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Concentration: ~0.1mg/ml Form: Supplied as a lyophilized powder in 20mM Tris-HCl, pH 8.0, 5mM MgCl2, 10% glycerol. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Effect of early luteal phase administration of a single dose mifepristone on immunohistochemical distribution of interleukin 1 alpha (IL-1 alpha) and transforming growth factor beta 1 (TGF-beta 1) in mid-luteal phase ovary of the rhesus monkey. Sengupta S, Ghosh D, Indian J Physiol Pharmacol 2003 Apr;47(2):221-4 2. [Analysis on association between the polymorphisms in apolipoprotein E, interleukin-1 alpha genes and Alzheimer's disease in Chengdu area] Tang MN, Zhang ZX, Liu XH, Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2004 Apr;21(2) :176-8 3. [Content of tumor necrosis factor alpha and interleukin 1-alpha in patients with acute purulent pleural cavity] Snizhko SS, Klin Khir 2003 Dec;(12):26-8 4. Lack of association between the interleukin-1 alpha (-889) polymorphism and early-onset Parkinson's disease. Moller JC, Depboylu C, Lohmuller F, Paus S, Neurosci Lett 2004 Apr 15;359(3):195-7 5. The paracrine role played by interleukin-1 alpha in the testis. Svechnikov K, Petersen C, Wahlgren A, Soder O, Curr Drug Targets Immune Endocr Metabol Disord 2004 Mar;4(1):67-74 6. Imiquimod-induced interleukin-1 alpha stimulation improves barrier homeostasis in aged murine epidermis. Barland CO, Zettersten E, Ye J, J Invest Dermatol 2004 Feb;122(2):330-6.
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