Technical Data
I8426-14
Interleukin 4 (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pitrakinra, T Cell Grow
Description:
IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Intracellular staining. Other applications have not been tested.

Recommended Dilution:
Direct ELISA: 1:5000 dilution will yield 0.4 O.D. using IgG (AP) Mab Rabbit x Mouse.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies
I1904-65D IgG (AP) Mab Rabbit x Mouse

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15C64Highly Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouseRat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Recombinant mouse Interleukin 4.
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with sterile dH2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Specificity:
Recognizes mouse Interleukin 4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Skeletal Muscle Pump vs. Respiratory Muscle Pump: Modulation of Venous Return from the Locomotor Limb in Humans. Miller JD, Pegelow DF, Dempsey JA, J Physiol 2005 Jan 13; 2. Evidence for enhanced bioavailability of trace elements in the marine ecosystem of Deception Island, a volcano in Antarctica. Deheyn DD, Gendreau P, Latz MI, Mar Environ Res 2005 Jul;60(1):1-33 3. Heart rate variability fraction-a new reportable measure of 24-hour R-R interval variation. Sosnowski M, Clark E, Macfarlane PW, Ann Noninvasive Electrocardiol 2005 Jan;10(1):7-15 4. High-power parametric amplification of 11.8-fs laser pulses with carrier-envelope phase control. Zinkstok RT, Witte S, Eikema KS, Opt Lett 2005 Jan 1;30(1):78-80 5. Passively mode-locked 914-nm Nd:YVO4 laser. Schlatter A, Krainer L, Paschotta R, Opt Lett 2005 Jan 1;30(1):44-6 6. Multidimensional, mapping-based complex wavelet transforms. Fernandes FC, van Spaendonck RL, IEEE Trans Image Process 2005 Jan;14(1):110-24.