Technical Data
Interleukin 4 (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pitrakinra, T Cell Grow
IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Intracellular staining. Other applications have not been tested.

Recommended Dilution:
Direct ELISA: 1:5000 dilution will yield 0.4 O.D. using IgG (AP) Mab Rabbit x Mouse.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies
I1904-65D IgG (AP) Mab Rabbit x Mouse

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG15C64Highly Purified
500ug-20CBlue IceMouseRat
~1mg/ml (after reconstitution)
Recombinant mouse Interleukin 4.
Purified by Ion Exchange chromatography.
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with sterile dH2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Recognizes mouse Interleukin 4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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