		Technical Data
I8428-02L	Interleukin 6 (IL-6, B cell Stimulatory Factor 2, BSF2, Cytotoxic T cell Differentiation Factor, CTL Differentiation Factor, CDF, Hepatocyte Stimulating Factor, HSF, Hybridoma Growth Factor, HGF, Hybridoma Plasmacytoma Growth Factor, HPGF, Interferon beta

Description:
IL-6 is a cytokine with a wide variety of biological functions. It plays an essential role in the final differentiation of B-cells into Ig-secreting cells. It induces myeloma and plasmacytoma growth, nerve cells differentiation in hepatocytes, and acute phase reactants.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Neutralization and RIA. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000-1:5000
Western Blot: 1:500-1:2000 Detects IL-6 in supernatants and lysates of 2x10e6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PMBC are stimulated for 24 hours with 1% human serum plus 10ng/ml E. coli LPS. Multiple bands bands will appear due to the variable amounts of glycosylation on the molecule (21-21kD).
Immunoprecipitation: 1:400-1:800; Precleaning with a non-specific rabbit IgG reduces background.
Immunohistochemistry (Frozen, paraffin): 1:400-1:800
Neutralization: 1:400. Incubate with sample for at least 4 hours before testing. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.
RIA: 1:4000-1:8000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not Determined

Immunogen:

Recombinant corresponding to human IL-6 expressed in E. coli. Cellular Localization: Secreted

Purity:

Serum

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

Specificity:

Recognizes native and recombinant human IL-6. Species Crossreactivity: mouse, porcine and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Silvera, M.R., et al., Lymphokine Cytokine Res 13: 277-285 (1994). 2. Novick, D., et al., Cytokine 4: 6-11 (1992). 3. Novick, D., et al., Hybridoma 8: 561-567 (1989). 4. Bottino, R., et al., Diabetes 53: 2559-2568 (2004).