Technical Data
Interleukin 6 (IL-6, B cell Stimulatory Factor 2, BSF2, Cytotoxic T cell Differentiation Factor, CTL Differentiation Factor, CDF, Hepatocyte Stimulating Factor, HSF, Hybridoma Growth Factor, HGF, Hybridoma Plasmacytoma Growth Factor, HPGF, Interferon beta
IL-6 is a cytokine with a wide variety of biological functions. It plays an essential role in the final differentiation of B-cells into Ig-secreting cells. It induces myeloma and plasmacytoma growth, nerve cells differentiation in hepatocytes, and acute phase reactants.

Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Neutralization, RIA, and Immunofluorescence/Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000-1:5000
Western Blot: 1:500-1:2000 Detects IL-6 in supernatants and lysates of 2x10e6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PMBC are stimulated for 24 hours with 1% human serum plus 10ng/ml E. coli LPS. Multiple bands bands will appear due to the variable amounts of glycosylation on the molecule (21-21kD).
Immunoprecipitation: 1:400-1:800; Precleaning with a non-specific rabbit IgG reduces background.
Immunohistochemistry (Frozen, paraffin): 1:400-1:800
Neutralization: 1:400. Incubate with sample for at least 4 hours before testing. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.
RIA: 1:4000-1:8000
Immunofluorescence (IC): 1:10-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
200ul-20CBlue IceHumanRabbit
Not Determined
Recombinant corresponding to human IL-6 expressed in E. coli. Cellular Localization: Secreted
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Recognizes native and recombinant human IL-6. Species Crossreactivity: mouse, rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Silvera, M.R., et al., Lymphokine Cytokine Res 13: 277-285 (1994). 2. Novick, D., et al., Cytokine 4: 6-11 (1992). 3. Novick, D., et al., Hybridoma 8: 561-567 (1989). 4. Bottino, R., et al., Diabetes 53: 2559-2568 (2004).