Technical Data
I8428-16F
Interleukin 6 (IL-6)
Description:
IL-6 is a pluripotent 20-22kD cytokine which plays a role in the pathophysiology of severe infection and regulates the immune response, acute phase reaction and haematopoiesis. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. Continuous IL-6 gene expression makes an essential contribution to a multistep oncogenesis of plasma cell neoplasia. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas.

Applications:
Suitable for use in Functional Studies, ELISA, Neutralization, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: Use as a coating antibody.
Immunohistochemistry: Frozen and Paraffin
Neutralization: Dilutions have to be made according to the amount of IL-6 to be inactivated. Before use, I8428-16F must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16A579Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Mouse Interleukin 6 (IL-6)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.
Specificity:
Recognizes mouse interleukin 6 (IL-6). Does not react with LPS (the component of the bacterial cell wall which is considered responsible for the toxicity of gram-negative bacteria) or TNF-alpha. Does not react with human or rat IL-6. Inhibits IL-6 induced proliferation of the B9 assay.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Takatsuki, F et al; J Immunol 1988, 141: 3072. 2. McKenzie, D et al; J Immunol 1988, 141: 2907. 3. van Snick, J et al; Annu Rev Immunol 1990, 8: 253. 4. Heinrich, PC et al; Biochem J 1990, 265: 621. 5. Starnes, HF Jr et al; J Immunol 1990, 145: 4185. 6. Hirano, T et al; Int J Cell Cloning 1991, 9: 166. 7. Hooghe-Peters, E et al; Pathol Res Pract 1991, 187: 622. 8. Litton, et al., Am J Pathol. 1997 May; 150(5): 16071618. 9. Whiteland et al., J Immunol Methods. 1997 Dec P5;210(1):103-8.