Technical Data
I8428-39B1
IL6R, Soluble, Recombinant, Human, Western Blot Control
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
The IL-6 receptor complex is composed of two membrane glycoproteins: the low affinity receptor and the signaltransducing component. The soluble form of IL6R is found in the urine of healthy adult humans and the serum of HIV positive individuals, as well as in the cell culture supernatants of stimulated PBMCs. This soluble form of IL6R results from either proteolytic cleavage from the membrane, or an isoform derived splice variant.The IL-6 receptor complex is composed of two membrane glycoproteins: the low affinity receptor and the signaltransducing component. The soluble form of IL6R is found in the urine of healthy adult humans and the serum of HIV positive individuals, as well as in the cell culture supernatants of stimulated PBMCs. This soluble form of IL6R results from either proteolytic cleavage from the membrane, or an isoform derived splice variant.

Source:
Recombinant corresponding to aa20-357 from the mature IL6- sR protein, fused to 6x His-tag at N-terminal, expressed in E. coli.

Applications:
Suitable for use in Western Blot as a control. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: Purified (SDS-PAGE)
Concentration: As reported
Form: Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kattan, MW. et al., (2003) J Clin Oncol. 21(19): 3573-9.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.