Technical Data
I8428-39M
Interleukin 6 Receptor (CD126, IL-6R)
Description:
IL-6 R, also known as CD126, is a transmembrane protein that associates with the signal-transducing subunit gp130 to form a high-affinity receptor complex for IL-6. It is expressed on T cells, activated B cells, and peripheral monocytes. A soluble form of IL-6 R can be released by proteolytic cleavage.

Applications:
Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 2.5ug/10e6 cells using mouse CD3+ splenocytes
Immunocytochemistry: 8-25ug/ml using immersion fixed mouse splenocytes
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a8H130Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa20-357 from mouse IL-6R expressed in NSO cells (P22272).
Purity:
Purified by Protein G affinity chromatography
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity:
Recognizes mouse IL-6R. Does not crossreact with rhIL-6R.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.