Technical Data
I8428-39M
Interleukin 6 Receptor (CD126, IL-6R)
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Technical Data |
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I8428-39M |
Interleukin 6 Receptor (CD126, IL-6R) |
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Description: IL-6 R, is a transmembrane protein that associates with the signal-transducing subunit gp130 to form a high-affinity receptor complex for IL-6. It is expressed on T cells, activated B cells, and peripheral monocytes. A soluble form of IL-6 R can be released by proteolytic cleavage. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml; 10ul label 1-2.5 x 10e5 cells in a total reaction volume < 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0ug/ml; The detection limit for is ~5ng/well. Western Blot: 1-2ug/ml; Using a colorimetric detection system, the detection limit is ~5 ng/lane and ~50 ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Optimal dilutions to be determined by the researcher. Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Interleukin-6 Receptor (rmIL-6 R) extracellular domain. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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