Technical Data
I8430-03L
Interleukin 8 (IL-8, 3-10C, AMCF-I, b-ENAP, CXCL8, C-X-C Motif Chemokine 8, Emoctakin, GCP1, GCP-1, Granulocyte Chemotactic Protein 1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, Monocyte-derived Neutrophil-activating Peptide, Monocyte-derived Neutrophil chemot
Description:
IL-8, also known as CXCL8, is produced by a range of cells including stimulated monocytes, mast cells, endothelial cells, keratinocytes and fibroblasts. Human IL-8 promotes neutrophil chemotaxis and degranulation.

Applications:
Suitable for use in ELISA, Western Blot, Neutralization Studies and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-2ug/ml; Use at 100ul/well, detects 0.2-0.4ng human IL-8/well.
Western Blot: 0.1-0.2ug/ml detects 1.5-3.0ng human IL-8
per lane under reducing and non-reducing conditions.
Neutralization Studies: 2-3ug/ml is found to yield half-maximal inhibition of the biological activity of 100ng/ml human IL-8.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Highly purified (>98% pure) recombinant protein corresponding to 17aa of human endothelial IL-8.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Specificity:
Recognizes all forms of natural and recombinant human IL-8.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Melhem, M.F., et al., Blood 82(7): 2038-2044 (1993). 2. Wen, Y., et al., Cancer Research 61(19): 7142-7147 (2001) (cites the use of this antibody in immunohistochemistry with paraffin embedded sections). 3. Nixon, C.S., et al., Infection and Immunity 68(9): 5284-5292 (2000) (cites the use of this antibody in ELISA). 4. Jung, Y.D., et al., Cytokine 18(4): 206-213 (2002) (cites the use of this antibody in Western Blot analysis).