Technical Data
I8432-11
Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
2ug
10ug
10ug
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Technical Data |
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I8432-11 |
Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF) |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. Human IL-10 is active on murine cells. Murine IL-10 is inactive on human cells. Recombinant human IL-10 is an 18.6kD protein with 161 amino acid residues. AA Sequence: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N Biological Activity: Determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED50 was found to be 2ng/ml, corresponding to a specific activity of 5x10e5 units/mg. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA |
Source: Human, recombinant expressed in E. coli Purity: 98% by SDS-PAGE and HPLC Endotoxin: 0.1ng/ug Form: Supplied as a lyophilized powder from 5mM sodium phosphate, pH 7.2, without additives. Reconstitute with 5mM Tris, pH 7.2 to a concentration of 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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