		Technical Data
I8434-27F	IL12B (Interleukin-12 Subunit beta, IL-12B, Cytotoxic Lymphocyte Maturation Factor 40kD Subunit, CLMF p40, IL-12 Subunit p40, NK Cell Stimulatory Factor Chain 2, NKSF2)

Description:
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:16,000
Western Blot: 0.1-0.3ug/ml, observed in human liver lysates on ~37kD bands
Immunohistochemistry (Paraffin): 4-6ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-QGKSKREKKDRVFTD, from IL12B, at the internal region of the protein (NP_002178.2).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes human IL12B.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Sheth K, De A, Nolan B, Friel J, Duffy A, Ricciardi R, Miller-Graziano C, Bankey P. Heat shock protein 27 inhibits apoptosis in human neutrophils. J Surg Res. 2001 Jul;99(1):129-33.