Technical Data
I8434-29E
IL12A (Interleukin-12 Subunit alpha, IL-12A, Cytotoxic Lymphocyte Maturation Factor 35kD Subunit, CLMF p35, IL-12 Subunit p35, NK Cell Stimulatory Factor Chain 1, NKSF1) (Carboxyfluorescein)
Description:
Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3. In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation. Human FoxP3+ Tregs do not constitutively express IL-35, but expression may be induced by activated dendritic cells.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommened Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Carboxyfluorescein conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG111C30Affinity Purified
SizeStorageShippingSourceHost
100Tests-20°CBlue IceHumanMouse
Concentration:
~0.02mg/ml
Immunogen:
Sf 21-derived, recombinant human IL-12 p35 subunit (rhIL-12 p35; aa23-219; Accession # P29459).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in sodium chloride, 0.5% BSA, 0.1% sodium azide. Labeled with Carboxyfluorescein.
Specificity:
Recognizes human IL-12 p35.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248. 2.Collison, L.W. et al. (2007) Nature 450:566. 3.Bardel, E. et al. (2008) J. Immunol. 181: 6898. 4.Seyerl, M. et al. (2010) Eur. J. Immunol. 40: 321.