Technical Data
IL12A (Interleukin-12 Subunit alpha, IL-12A, Cytotoxic Lymphocyte Maturation Factor 35kD Subunit, CLMF p35, IL-12 Subunit p35)
Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40kD (p40) subunit and a 35kD (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-g and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL-12 share 70-60aa sequence identity in their p40 and p35 subunits, respectively.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2A11n102Affinity Purified
100ug-20CBlue IceMouseRat
~0.5mg/ml (after reconstitution)
Recombinant protein corresponding to aa23-215 from mouse IL12A expressed in Sf21 cells (NP_032377).
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes mouse IL12A. Species crossreactivity: recombinant mouse IL-12 p35+p40 heterodimer, recombinant rat IL-12 p35 (50%). Does not react with recombinant human IL-12 p35, recombinant mouse IL-12 p40, IL-23 p19+p40 heterodimer, recombinant canine IL-12 p35+p40 heterodimer, recombinant feline IL-12 p35+p40 heterodimer.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.