Technical Data
I8435-05
Interleukin 13 (IL-13)
Description:
IL-13 is an immunoregulatory cytokine produced mainly by T helper 2 cells, but also by mast cells and NK cells. IL-13 is a unique cytokine having structural features and functional characteristics with IL-4. However, it only shares about 30% homology with the IL-4 sequence. IL-13 inhibits IL-1 beta, IL-6, IL-8, and TNF-alpha resulting in anti-in ammatory effects on monocytes and macrophages. It also induces B cells to proliferate and isotype-switch to the production of IgE. In addition, IL-13 modulates TH2 cell development interfering key roles in the ostracizing of gastrointestinal parasites and in the evolvement of asthma and allergy.

Applications:
Suitable for use in Neutralizing, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 5ug/ml. Detects 100ng of human IL-13
Immunohistochemistry: Frozen sections
Note: Further dilutions should be made in in medium or buffered solutions with a carrier protein such as 1% BSA.
Optimal dilutions to be determined by researcher.

Hybridoma:
Rat spleen cells fused with P3X63-AG8.653 myeloma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11.B2.1Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRat
Concentration:
~1mg/ml
Immunogen:
Purified recombinant human IL-13
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a liquid in PBS.
Specificity:
Recognizes natural and recombinant human IL-13.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pawanakar, R.U., et al., Amer. J. Resp. Critical Care Med. 152: 2059-2067 (1995). 2. Prussin C, Metcalf D., I. Immunol. Meth. 188: 117-128 (1995).