Technical Data
I8439-02B
Interleukin 17, Recombinant, Rat (IL-17, CTLA8, IL-17A, Interleukin-17A precursor, Cytotoxic T-lymphocyte-associated antigen 8)
5ug
25ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Interleukin-17A (IL-17A), also known as CTLA-8, is a pro-inflammatory cytokine member of a six-species family of proteins (IL-17A-17F). IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most importantly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. Human, mouse and rat IL-17A show activity on mouse cells. Recombinant rat IL-17A is a non-glycosylated, disulfide-joined homodimer, having a total molecular mass of 30kD.

Biological Activity:
The activity is determined by the dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts and is typically 1-2ng/ml.

Amino Acid Sequence:
MAVLIPQSSV CPNAEANNFL QNVKVNLKVL NSLSSKASSR RPSDYLNRST SPWTLSRNED PDRYPSVIWE AQCRHQRCVN AEGKLDHHMN SVLIQQEILV LKREPEKCPF TFRVEKMLVG VGCTCVSSIV RHAS

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
30.0kD
Source: E. coli
Purity: ~95% Analysis by RP-HPLC; Reducing and non-reducing SDS-PAGE. Endotoxin: 0.1ng/ml.

Form: Supplied as a lyophilized powder from 10mM sodium citrate, pH 3.0. Reconstitute with sterile dH2O to a concentration of 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.