Technical Data
I8439-97G
IL18 (Interleukin-18, Iboctadekin, Interferon gamma-inducing Factor, IFN-gamma-inducing Factor, Interleukin-1 gamma, IL-1 gamma, IGIF, IL1F4)
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Technical Data |
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I8439-97G |
IL18 (Interleukin-18, Iboctadekin, Interferon gamma-inducing Factor, IFN-gamma-inducing Factor, Interleukin-1 gamma, IL-1 gamma, IGIF, IL1F4) |
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Description: IL-18, initially described as IFN-g inducing factor (IGIF), is a pro-inflammatory cytokine. It is an 18kD cytokine which identified as a costimulatory factor for production of interferon-y (IFN-y) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24kD molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. The cytokine IL-18 possesses pleiotropic biological properties such as activation of NF-kb, Fas ligand expression, induction of both CC and CXC chemokines, enhancement of the production of IFN-g and GM-CSF, and induction of CD4+ cytolytic T cell response. Its role in neurodegeneartion disease has also been reported. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-4ug/ml Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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