Technical Data
IL18 (Interleukin-18, Iboctadekin, Interferon gamma-inducing Factor, IFN-gamma-inducing Factor, Interleukin-1 gamma, IL-1 gamma, IGIF, IL1F4)
IL-18, initially described as IFN-g inducing factor (IGIF), is a pro-inflammatory cytokine. It is an 18kD cytokine which identified as a costimulatory factor for production of interferon-y (IFN-y) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24kD molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. The cytokine IL-18 possesses pleiotropic biological properties such as activation of NF-kb, Fas ligand expression, induction of both CC and CXC chemokines, enhancement of the production of IFN-g and GM-CSF, and induction of CD4+ cytolytic T cell response. Its role in neurodegeneartion disease has also been reported.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to 41aa of human IL-18.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Recognizes human IL18.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.