Technical Data
IL-32 (Interleukin-32, Natural Killer Cells Protein 4, Tumor Necrosis Factor alpha-inducing Factor, IL32, NK4, TAIF)
IL-32 is a 234aa member of the cytokine family which plays an important role in innate and adaptive immune responses. It contains a tyrosine sulfation site, three potential N-myristoylation sites, multiple putative phosphorylation sites and a RGD cell-attachment sequence. IL-32 shows increased expression after activation of T lymphocytes and natural killer cells by IL-2 or mitogens. Human IL-32 exists as four splice variants. Reports suggest that it activates typical cytokine signal pathways of nuclear factor-kappa B (NF-kappaB) and p38 mitogen-activated protein kinase. It induces the production of various cytokines such as TNFA/TNF-alpha, macrophage inflammatory protein (MIP)-2 and IL-8, and has a possible role in inflammatory:autoimmune diseases like chronic obstructive pulmonary disease, Crohn's disease and psoriasis. It is ubiquitously expressed in most tissues with selective expression in lymphocytes and more prominently in immune cells.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human lymph node lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa200-234 of human IL-32.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Recognizes human Interleukin 32.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kim, SH. et al. Immunity. 22:131-142 (2005). 2. Goda, C. et al. Int. Immunol. 18:233-240 (2006).