Technical Data
I8442-42S
IL-32 (Interleukin-32, Natural Killer Cells Protein 4, Tumor Necrosis Factor alpha-inducing Factor, IL32, NK4, TAIF)
Description:
IL-32 is a 234aa member of the cytokine family which plays an important role in innate and adaptive immune responses. It contains a tyrosine sulfation site, three potential N-myristoylation sites, multiple putative phosphorylation sites and a RGD cell-attachment sequence. IL-32 shows increased expression after activation of T lymphocytes and natural killer cells by IL-2 or mitogens. Human IL-32 exists as four splice variants. Reports suggest that it activates typical cytokine signal pathways of nuclear factor-kappa B (NF-kappaB) and p38 mitogen-activated protein kinase. It induces the production of various cytokines such as TNFA/TNF-alpha, macrophage inflammatory protein (MIP)-2 and IL-8, and has a possible role in inflammatory:autoimmune diseases like chronic obstructive pulmonary disease, Crohn's disease and psoriasis. It is ubiquitously expressed in most tissues with selective expression in lymphocytes and more prominently in immune cells.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human lymph node lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa200-234 of human IL-32.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human Interleukin 32.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kim, SH. et al. Immunity. 22:131-142 (2005). 2. Goda, C. et al. Int. Immunol. 18:233-240 (2006).