Technical Data
I8442-43
Interleukin 32 alpha, Recombinant, Human (IL-32a, Interleukin-32 Precursor, Natural Killer Cells Protein 4, NK4, TAIF, TAIFa, Tumor Necrosis Factor alpha-inducing Factor)
2ug
10ug
100ug
Molecular Biology Storage: -20°CShipping: Blue Ice
Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-a, and MIP-2 production from human & mouse macrophage cell lines. It is upregulated in activated T- & NK-cells, and IFN-g-treated epithelial cells.

Endotoxin: 0.1EU/ug

Biological Activity:
Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50 is in the range of 0.125-1ug/ml.

AA Sequence:
MCFPKVLSDD MKKLKARMHQ AIERFYDKMQ NAESGRGQVM SSLAELEDDF KEGYLETVAA YYEEQHPELT PLLEKERDGL RCRGNRSPVP DVEDPATEEP GESFCDKSYG APRGDKEELT PQKCSEPQSS K

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile water containing 0.1% HSA or BSA. Reconstituted product is stable for 6 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
14.9kD
 Source: Recombinant, human, produced in E. coli
Purity: 97% as determined by reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder from 50mM sodium phosphate, pH 7.5. Reconstiute with sterile water containing 0.1% HSA or BSA to a concentration of 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.